MICROBIOLOGY AND VIROLOGY

2011 : Finished Medical University of Sofia- Bulgaria , Specialty Doctor of Medicine\r\n2009-2012 : worked in Emergency Department in “Prevent DCC” hospital\r\n2010-2013 : intern in Dermatology Department in “Tokuda Hospital” in Sofia Bulgaria\r\n2013-2014 : Intern in Microbiology Department in “ISUL- TSARITSA IOANNA” hospital in Sofia Bulgaria\r\n2014-2018 : Specialist Microbiologist in the Department of Medical Microbiology in Medical University Sofia - Bulgaria\r\n2016- until today : Assistant Professor in the Department of Medical Microbiology in Medical University Sofia – Bulgaria\r\n2016-2019 : \r\nI am fluent in Greek , English and Bulgarian language\r\n

Invasive infections caused by methicllin resistant Staphylococcus aureus and coagulaso-negative staphylococci (MRSA/MRSCoN) require fast laboratory detection and start of adequate treatment. \r\nThe aim of this study was to develop a new faster protocol for direct detection of MRSA/MRSCoN in blood cultures and in abscess punctures. For this purpose were used polymerase-chain reaction (PCR) by primers for species specific identification of S. aureus and methicllin resistance gene mecA. We examined 85 growth-positive BACTEC blood cultures and 56 abscess punctures by routine microbiological assay and simultaneous PCR detection of MRSA/MRSCoN. The specificity of the PCR was evaluated by using DNA from another 16 microbial species for negative controls. We determined the susceptibility to methicillin by disc cefoxitine according EUCAST 2019 criteria and minimum inhibitory concentration (MIC) of oxacillin against the S. aureus isolates using the E-test. In the blood cultures, the two methods detected near 40% MRSA, resp.94% MRCoNS. In the punctures, the PCR assay identified near 20% MRSA. The PCR and the routine microbiological results for the blood samples are fully consistent but the new method was faster (only a few hours were need). Among the punctures, there were five PCR MRSA positive and culture negative samples. The new PCR protocol was more sensitive and again faster for detecting MRSA from abscess punctures than the routine microbiological techniques. This molecular -genetic test will speed up the right choice of empirical therapy, which is extremely important for saving patients’ lives.\r\n

My name is Khashaiar Mansouri. I am 24 years old and currently studying Doctor of veterinary\r\nmedicine at the Islamic azad university Garmsar branch. My research focous is on zoonotic\r\ndiseases particulary Mycobacterium in Cat and Mice, as well as Bukholderia mallei in Guinea\r\npig.recently, I had a poster presentation in the 19th international and Iranian congress of\r\nmicrobiology.

Background and Aim:\r\nBovine tuberculosis is one of the most important zoonotic diseases in Bovidae. Humans and\r\nanimals that transit to the farm can transfer Mycobacterium to the cattle. Hence, the aim of this\r\nstudy is to evaluate the possible role of cats in transferring the Mycobacterium infection in dairy\r\nfarms.\r\nMethods:\r\nFrom a dairy cattle farm with more than 20 percent infection of Mycobacterium , seven cats were\r\ncaptured and their gastric juice cultured in the LJ and LG medium. The Acid-Fast staining of the\r\nisolates prepared to identify Mycobacterium and PCRs were carried out afterwards.\r\nResults:\r\nFive Out of seven cultures were positive in direct smear by Acid Fast staining and in PCR-\r\n16SrRNA, which indicates that the above-mentioned isolates belong to the Mycobacterium genus.\r\nAlso, positive PCR-IS6110 confirmed that the isolate species are identified as Mycobacterium\r\ntuberculosis Complex. Currently, we are conducting Sequencing for the exact identification of\r\nthese isolates.\r\nConclusion:\r\nAnimals such as mice and cats that live in the farm can harbor Mycobacterium. In this study, it\r\nhas been proven that cats certainly transfer Mycobacterium to the cattle farms.\r\nKeywords:\r\nMycobacterium tuberculosis Complex, Mycobacterium , PCR IS6110, 16SrRNA

Shuhong Luo is a Professor of Microbiology at the Department of Laboratory Medicine,\r\nInternational Conference on\r\nFibromyalgia and Chronic Pain (June 15-16, 2016 Philadelphia,\r\nUSA)\r\nFibromyalgia 2016\r\nJune 15-16, 2016\r\nFoshan University. He has completed his BSc and MSc from Xiangya Medical College, Central South University, and PhD from Nanjing Medical University. His research areas include parasite and viral pathogenesis, molecular mechanisms of pathogen virulence, host interactions, Tumor biomarker screening and identification for early detection.

Oncolytic viral therapies against cancers, using variously attenuated or recombinant viruses, have appeared as a promising method in cancer treatment in recent years. Orf virus (ORFV) strain NZ2 has been shown to have antitumor effects in animal models mediated by immunoregulation profile, however, little is known about the molecular cellular mechanism of orf virus’s anti-cancer effect. Here we report ORFV strain NA1/11, isolated from a sheep in Jilin province of China, inhibited the growth of colorectal cancer (CRC) cells lines including Caco-2, HCT116, LoVo, RKO, SW480, SW1116 cells. ORFV strain NA1/11 also significantly inhibited the growth and the pulmonary metastasis of CRC cells in vivo. The inhibitory mechanism of ORFV strain NA1/11 involved apoptosis and autophagy induction. Besides, we utilized a cytokine antibody array to develop a more comprehensive description of the cytokines by ORFV, which indicated that ORFV likely plays roles in the regulation of key factors relevant to apoptosis, autoimmunity/inflammation, angiogenesis and the cell cycle for further molecular mechanism studies. These results suggested that ORFV could be an oncolytic virus for CRC therapy.\r\nRecent Publications\r\n1. Ruixue Wang, Yong Wang, Fang Liu, Shuhong Luo (2018). Orf virus: a promising new therapeutic\r\nagent. Revin Med Virol. 1(9): e2013.\r\n2. Ruixue Wang and Shuhong Luo (2018) Orf virus: a new class of immunotherapy drugs.System Biology.\r\n3. Mingjian Long, Yuanyuan Wang, Daxiang Chen, Yong Wang, Ruixue Wang, Daoyuan Gong, Haijian He, Daniel L Rock, Wenbo Hao, Shuhong Luo (2018) Identification of host cellular proteins LAGE3 and IGFBP6 that interact with orf viral protein ORFV024; Gene 661, 2018, 60-67.\r\n4. Wei Li, Huiqin Chen, Hao Deng, Zhenzhan Kuang, Mingjian Long, Daxiang Chen, Xiaoqing Liao, Ming Li, Daniel L.Rock, Shuhong Luo, Wenbo Hao (2018) Orf virus encoded protein ORFV119 induces cell apoptosis through the extrinsic and intrinsic pathways. Front Microbiol 9: 1056.\r\n5. Sushil Khatiwada, Gustavo Delhon, Ponnuraj Nagendraprabhu, Sabal Chaulagain, Shuhong Luo, Diego G Diel, Eduardo F Flores, Daniel L Rock L (2017) A parapoxviral virion protein inhibits NF-κB signaling early in infection. PLoS Pathog 13(8): e1006561.